"presentations" "Title(English)","Title(Japanese)","Presenter(s)(English)","Presenter(s)(Japanese)","Conference name(English)","Conference name(Japanese)","Presentation date","Invited talk","Language","Presentation type","Organizer(English)","Organizer(Japanese)","Venue(English)","Venue(Japanese)","URL","Description(English)","Description(Japanese)" "Praja 1 E3 ubiquitin ligase suppresses adenovirus-induced neuronal TDP-43 aggregate","Praja 1 E3 ubiquitin ligaseによる培養ニューロン細胞質TDP-43凝集体の形成抑制","渡部和彦, 加藤陽一郎, 佐久間美帆, 村田麻喜子, 新井田素子, 竹村 太郎, 花方 信孝, 他田真里, 柿田明美, 柴田亮行","渡部和彦, 加藤陽一郎, 佐久間美帆, 村田麻喜子, 新井田素子, 竹村 太郎, 花方 信孝, 他田真里, 柿田明美, 柴田亮行","第61回日本神経学会学術大会","第61回日本神経学会学術大会","2020-08-31","","eng","oral_presentation","","","","","","Transactive response DNA-binding protein-43 (TDP-43) is a main constituent of cytoplasmic aggregates in neuronal and glial cells in cases of amyotrophic lateral sclerosis (ALS). We have previously demonstrated neuronal cytoplasmic aggregate formation induced by recombinant adenoviruses expressing human wild type (WT) and C-terminal fragment (CTF) TDP-43 under the condition of proteasome inhibition. These TDP-43 aggregate formation was markedly suppressed by co-infection of an adenovirus expressing heat shock transcription factor 1 (HSF1), a master regulator of heat shock response. In the present study, we performed DNA microarray analysis to identify candidate molecules locating downstream of HSF1 that counteract TDP-43 aggregate formation. Methods: The 1464R rat neural stem cells were differentiated into neurons in the presence of retinoic acid, and co-infected with adenoviruses expressing WT and CTF TDP-43, and HSF1 in the presence of proteasome inhibitor MG-132. Total RNAs were then isolated and DNA microarray analysis was performed, and candidate genes were cloned and examined. Results: We found 64 genes that are upregulated more than 2-fold by HSF1 transduction, and 393 genes that are upregulated more than 2-fold in association with aggregate inhibition by HSF1 transduction. In these, Praja 1 RING-finger E3 ubiquitin ligase (PJA1) suppressed phosphorylation and aggregate formation of TDP-43. Conclusion: PJA1 upregulated by HSF1 has a capability of TDP-43 phosphorylation and aggregation, which may have therapeutic potential in ALS.","" "Construction of “PoLyInfo RDF” database for practical material development (II):Open data-driven by using data linking with PubChem RDF","実用材料開発のためのデータベース「PoLyInfo RDF」の構築 (II):PubChem RDFとの統合による境界なきデータ駆動","石井 真史, 竹村 太郎, 谷藤 幹子","石井 真史, 竹村 太郎, 谷藤 幹子","2020年第67回春季学術講演会","2020年第67回春季学術講演会","2020-03-12","","jpn","oral_presentation","","","","","","","材料開発では、基礎物性以外の要素、安全性や競合特許も考慮する必要がある。セマンティックウェブ技術に基づき、物質・材料研究機構の高分子データベースPoLyInfoの重合情報をRDF化し,バイオアッセイなどの情報を持つNIHのPubChemデータベースとの概念統合に、161,640のRDF Tripleを使って成功した。更に、新たにPoLyInfoの71,615件の重合情報を整理し統合強化を図った。" "培養ニューロン細胞質TDP-43凝集体形成を抑制する分子の解析","培養ニューロン細胞質TDP-43凝集体形成を抑制する分子の解析","渡部 和彦, 加藤陽一郎, 佐久間美帆, 村田麻喜子, 新井田(川口)素子, 竹村 太郎, 花方 信孝, 柿田明美, 柴田亮行","渡部 和彦, 加藤陽一郎, 佐久間美帆, 村田麻喜子, 新井田(川口)素子, 竹村 太郎, 花方 信孝, 柿田明美, 柴田亮行","第14回日本臨床ストレス応答学会大会","第14回日本臨床ストレス応答学会大会","2019-11-02","","jpn","oral_presentation","","","","","","","筋萎縮性側索硬化症(ALS)は運動ニューロンの選択的な変性脱落により死に至る最も過酷な神経変性疾患であり,ニューロンにtransactive response DNA- binding protein 43 (TDP-43) 蛋白を含む細胞質凝集体が出現する.実験的にはTDP-43とそのC末断片を発現する組換えアデノウイルスをプロテアソーム阻害条件下で培養ニューロンに感染させると,細胞質TDP-43凝集体の形成とともに細胞死が惹起される.一方,熱ショック応答のマスター制御転写因子であるheat shock transcription factor 1 (HSF1) を発現する組換えウイルスの共感染により細胞質TDP-43凝集体形成が顕著に抑制される.本研究ではこの培養TDP-43細胞質凝集体形成モデルを用いてDNAマイクロアレイ解析によりHSF1の下流で働くTDP-43凝集抑制分子の同定を試みた.ラット神経幹細胞株1464R由来分化ニューロンにTDP-43, HSF1組換えウイルスを共感染させ,プロテアソーム阻害剤MG-132を負荷してTDP-43凝集体を形成させたのちtotal RNAを調製し,マイクロアレイ解析を行った.その結果,HSF1により2倍以上に発現が上昇する遺伝子を64個,HSF1による凝集体形成抑制に伴って2倍以上に発現が上昇する遺伝子を393個同定した.このうち50候補遺伝子の蛋白翻訳領域をEGFPベクターにクローニングし,一部は組換えウイルスを作製して蛍光観察およびウェスタンブロットで解析したところ,新たな機能分子としてPraja 1 (PJA1)ユビキチンリガーゼに顕著なTDP-43凝集体形成抑制効果を見出した.マイクロアレイ解析を手がかりとしてHSF1の下流に位置するTDP-43凝集体抑制因子を順次同定し,ALSの新規治療法の開発に繋げていきたい." "PoLyInfo RDF: A Semantically Reinforced Polymer Database for Materials Informatics","PoLyInfo RDF: マテリアルズインフォマティクスに向けた高分子データベースのセマンティックな強化","竹村 太郎, 石井 真史, 谷藤 幹子","竹村 太郎, 石井 真史, 谷藤 幹子","The International Semantic Web Conference","The International Semantic Web Conference","2019-10-26","","eng","oral_presentation","","","","","","For materials database integration, we introduce a semantic web technology into a polymer database, called PoLyInfo. A resource data framework (RDF) was used to create a semantic description of the polymer formation process (polymerization). The polymerization correlates polymers with their source monomers, and then the monomers in the PoLyInfo RDF were conceptually linked to those in other chemical substance databases, such as Nikkaji. Alt-hough common ontology was not used in the PoLyInfo and Nikkaji RDFs, 94.3% of the monomers in PoLyInfo were assigned a Nikkaji substance ID, and the established information path provided probable polymerization in-formation for monomers in the Nikkaji RDF.","" "Construction of “PoLyInfo RDF” database for practical material development:Crossover data-driven by using semantic web technology","実用材料開発のためのデータベース「PoLyInfo RDF」の構築:セマンティックウェブ技術による境界無きデータ駆動","石井 真史, 谷藤 幹子, 竹村 太郎","石井 真史, 谷藤 幹子, 竹村 太郎","第80回応用物理学会 秋季学術講演会","第80回応用物理学会 秋季学術講演会","2019-09-18","","jpn","oral_presentation","","","","","","","実際の材料開発においては、純粋な物理・化学的な興味以外の要素も考慮する必要がある。例えば、採算性、安全性、環境問題などが学術的興味以上に重要になり、またそれが逆に新しい材料開発における研究のモチベーションとなる。セマンティックウェブ技術は、こうした境界無き情報の流通を促す国際的取り組みであり、W3C(World Wide Web Consortium)の指針のもとで、次第に利用者が増えている。 この技術は、あらゆる情報を主語・述語・目的語の三種で記述するRDFと呼ばれるプロトコルにより実現される。現在、世界の巨大データベース(DB)の多くがRDFを取り入れており、化学分野では日化辞RDFやPubChem RDFなどがよく知られている。本研究では、NIMSの高分子DB、PoLyInfoの重合情報をRDF化し、原料モノマーを介して、既にRDF化している巨大DBとの統合を図った。" "Trial of remodeling of NIMS polymer database, PoLyInfo in RDF-The first report of semantic connection to unspecified databases-","NIMS高分子データベースPoLyInfoのRDF化-セマンティックなデータベース統合への試行報告-","竹村 太郎, 石井 真史, 谷藤 幹子","竹村 太郎, 石井 真史, 谷藤 幹子","サービスコンピューティング研究会","サービスコンピューティング研究会","2019-05-31","","jpn","oral_presentation","","","","","","","物質・材料研究機構の高分子データベースPoLyInfoの一部を試験的にRDF化し,有機化学でよく知られたJSTの日化辞やNIHのPubChemデータベースとの統合をシミュレートした.重合反応を接点にした情報共有の実証と併せ,本格的なセマンティックなデータ統合を進める上でPoLyInfoの構造上の課題を調査した.一般的にRDB(Relational Database)からRDFは比較的容易に生成できるが,PoLyInfoの場合,データベースの列名の設定やデータ記述の正規化において,RDF化にあたり幾つかの課題があることが明らかになった.独立した大規模な Web上の データベースやデータセットを関連付けるLinked Dataの手法を,データ駆動の材料科学に適用するにあたり,現状と解決すべき課題を報告する." "Apatite Nanoparticles-Assisted Dissolving Microneedles for Transdermal Adjuvant CpG DNA Delivery","Apatite Nanoparticles-Assisted Dissolving Microneedles for Transdermal Adjuvant CpG DNA Delivery","陳 民樺, 箕輪 貴司, Abigail Magee, リ コウラン, 竹村 太郎, 花方 信孝","陳 民樺, 箕輪 貴司, Abigail Magee, リ コウラン, 竹村 太郎, 花方 信孝","Advanced materials conference 2016, Malaysia","Advanced materials conference 2016, Malaysia","2016-11-28","","jpn","oral_presentation","","","","","","","Microneedle-mediated vaccines delivery holds a minimally invasive property to puncture the skin, followed by the diffusion of antigen into the skin. Dissolving microneedles with fast dissolution rates would be attractive for rapid delivery scenarios into the skin, such as vaccinations, where microneedles can be inserted, removed, and discarded without making the patient wait. However, microneedles for vaccine delivery is still the big challenge to find a safe and efficient delivery system to help genetic materials target to the specific cell. Thus, we investigate the use of apatite nanoparticles as the delivery agent loaded in dissolving microneedle patch. Apatite is the main mineral found in human bone and teeth and is considered as a novel non-viral vector for targeted gene delivery. The" "Dissolving Microneedles As a Transdermal Delivery System for Adjuvant CpG DNA","Dissolving Microneedles As a Transdermal Delivery System for Adjuvant CpG DNA","陳 民樺, 箕輪 貴司, リ コウラン, 竹村 太郎, 花方 信孝","陳 民樺, 箕輪 貴司, リ コウラン, 竹村 太郎, 花方 信孝","Advanced materials conference 2016, Malaysia","Advanced materials conference 2016, Malaysia","2016-11-28","","jpn","oral_presentation","","","","","",""," Microneedle-mediated vaccines delivery holds a minimally invasive property to puncture the skin, followed by the diffusion of antigen into the skin. Dissolving microneedles with fast dissolution rates would be attractive for rapid delivery scenarios into the skin, such as vaccinations, where microneedles can be inserted, removed, and discarded without making the patient wait. According to the Federal Food, Drug, and Cosmetic Act (FD&C Act) requirement, dissolving microneedles patch, made from drug-loaded dissolving polymeric matrices, is classified under the umbrella of “drug product”. Thus, the basis for the acceptance criteria for the development of microneedle patch should necessarily be safe and efficient. In this regard, we demonstrate a prototype of dissolving microneedle patch, w" "Dissolving Microneedles As a Transdermal Delivery System for Adjuvant CpG DNA","Dissolving Microneedles As a Transdermal Delivery System for Adjuvant CpG DNA","CHEN, Min-Hua, MINOWA, Takashi, LI, Xianglan, TAKEMURA, Taro, HANAGATA, Nobutaka","CHEN, Min-Hua, MINOWA, Takashi, LI, Xianglan, TAKEMURA, Taro, HANAGATA, Nobutaka","10th Anniversary International Symposium on Nanomedicine","10th Anniversary International Symposium on Nanomedicine","2016-11-24","","eng","oral_presentation","","","","","","Microneedle-mediated vaccines delivery holds a minimally invasive property to puncture the skin, followed by the diffusion of antigen into the skin. Dissolving microneedles with fast dissolution rates would be attractive for rapid delivery scenarios into the skin, such as vaccinations, where microneedles can be inserted, removed, and discarded without making the patient wait. According to the Federal Food, Drug, and Cosmetic Act (FD&C Act) requirement, dissolving microneedles patch, made from drug-loaded dissolving polymeric matrices, is classified under the umbrella of “drug product”. Thus, the basis for the acceptance criteria for the development of microneedle patch should necessarily be safe and efficient. In this regard, we demonstrate a prototype of dissolving microneedle patch, wh","" "Enhancing CpG-DNA delivery for cancer immunotherapy by calcium phosphate nanoparticles","Enhancing CpG-DNA delivery for cancer immunotherapy by calcium phosphate nanoparticles","Min-Hua Chen, 竹村 太郎, 箕輪 貴司, 生駒 俊之, 田中 順三, Feng-Huei Lin, 花方 信孝","Min-Hua Chen, 竹村 太郎, 箕輪 貴司, 生駒 俊之, 田中 順三, Feng-Huei Lin, 花方 信孝","The 5th Asian Biomaterials Congress","The 5th Asian Biomaterials Congress","2015-05-06","","jpn","oral_presentation","","","","","","","DNA that contains immunostimulatory cytosine-phosphate-guanosine (CpG) motifs is as a potent immunostimulator to induce innate and adaptive immune response through the triggering of Toll-like receptor 9 (TLR9), a molecule located in the endolysosomes of B cells and antigen-presenting cells. With the delivery of CpG-DNA, because the receptor TLR9 is located in the endolysome, CpG-DNA must be retained in the endolysosome for a long period of time. In this regard, calcium phosphate based nanoparticles has been considered as one of the suitable candidate for CpG-DNA delivery due to its pH-dependent solubility in acid endolysosomal environment, where followed by the release of DNA in endolysosome. However, calcium phosphate consists of two phases in the body in the form of a noncrystalline (amo" "Hydroxyapatite nanoparticles for enhancing CpG oligodeoxynucleotide delivery in macrophage for cancer immunotherapy","Hydroxyapatite nanoparticles for enhancing CpG oligodeoxynucleotide delivery in macrophage for cancer immunotherapy","Min-Hua Chen, 竹村 太郎, 箕輪 貴司, Feng-Huei Lin, 花方 信孝","Min-Hua Chen, 竹村 太郎, 箕輪 貴司, Feng-Huei Lin, 花方 信孝","7th World Congress on Preventive and Regenerative Medicine","7th World Congress on Preventive and Regenerative Medicine","2014-11-04","","jpn","oral_presentation","","","","","","","Synthetic oligodeoxynucleotides (ODNs) that contain immunostimulatory cytosine-phosphate-guanosine (CpG) motifs is as a potent immunostimulator to induce innate and adaptive immune response through the triggering of Toll-like receptor 9 (TLR9), a molecule located in the endolysomes of B cells and antigen-presenting cells (APCs). The immune cascade that is elicited by CpG ODNs results in the activation of natural killer cells and cytotoxic T lymphocytes that facilitates the treatment of cancer. With the delivery of CpG ODN, because the receptor TLR9 is located in the endosome, CpG ODNs must be retained in the endosome for a long period of time. Therefore, delivery system using CpG ODNs require a design strategy different from that of conventional drug delivery system. We believe that the in" "Fibroblast growth factor immobilized polyethylene porous scaffolds via mussel adhesive peptide and heparin for inducing angiogenesis","Fibroblast growth factor immobilized polyethylene porous scaffolds via mussel adhesive peptide and heparin for inducing angiogenesis","Yusuke SAKAI, Sachiro KAKINOKI, 竹村 太郎, 花方 信孝, Toshia FUJISATO, Tetsuji YAMAOKA","Yusuke SAKAI, Sachiro KAKINOKI, 竹村 太郎, 花方 信孝, Toshia FUJISATO, Tetsuji YAMAOKA","The 15th International Conference on Biomedical Engineering ","The 15th International Conference on Biomedical Engineering ","2013-12-04","","jpn","oral_presentation","","","","","","","he ultimate goal of tissue engineering is to repair and construct 3D tissues and organs. Porous materials have been investigated as a temporary extracellular matrix in regenerative medicine, but even a simple tissue has not been well reconstructed yet. The most crucial problem is the difficulty in inducing vascular network into caffolds. In this study, we developed the easy method for immobilizing fibroblast growth factor (FGF) on polyethylene porous scaffold (PEPS) using mussel adhesive peptide and heparin, and evaluated the angiogenesis within scaffolds in vivo. FGF was immobilized on PEPS with the pore size of 30 or 150 m by just immersing in three kinds of solutions. First, PEPS was immersed in the aqueous solution of peptide having oligo-lysine and mussel adhesive sequence (P" "細胞観察用ナノパーティクルの開発","細胞観察用ナノパーティクルの開発","竹村 太郎","竹村 太郎","分子 ・ 物質合成プラットフォーム技術研修会","分子 ・ 物質合成プラットフォーム技術研修会","2013-08-22","","jpn","oral_presentation","","","","","","","NIMS分子・物質合成プラットフォームにおける支援実態に関する発表。細胞観察用ナノパーティクルの開発を例に、細胞毒性評価やレーザー走査型共焦点蛍光顕微鏡観察など提供可能な当プラットフォームのサービスを紹介する。" "遺伝子マイクロアレイを用いたポリエチレン多孔質体に対するin vivoでの免疫系細胞の応答解析","遺伝子マイクロアレイを用いたポリエチレン多孔質体に対するin vivoでの免疫系細胞の応答解析","坂井勇亮, 柿木佐知朗, 竹村 太郎, 花方 信孝, 藤里俊哉, 石原一彦, 山岡哲二","坂井勇亮, 柿木佐知朗, 竹村 太郎, 花方 信孝, 藤里俊哉, 石原一彦, 山岡哲二","第59回高分子研究発表会","第59回高分子研究発表会","2013-07-12","","jpn","oral_presentation","","","","","","","3次元組織再生のために多孔質足場材料が広く研究され、生理活性のない合性高分子多孔質体より、コラーゲンなどで作製した多孔質では内部への組織浸潤が活発であると報告されている。しかしながらこの組織が、どのような生体応答に起因するかは明確でない。我々は、この主要因子を特定することが多孔質体を用いた3次元組織の再生に有用であると考え、孔径等が同じで表面が異なる多孔質体に対する生体応答を、免疫系細胞の活性化挙動を中心遺伝子レベルで解析した。" "Hydroxyapatite Coating prepared by solution treatment on AZ31 Magnesium Alloy","Hydroxyapatite Coating prepared by solution treatment on AZ31 Magnesium Alloy","廣本 祥子, 友澤 方成, 竹村 太郎, 花方 信孝, 丸山 典夫","廣本 祥子, 友澤 方成, 竹村 太郎, 花方 信孝, 丸山 典夫","WMRIF - 3rd International Workshop for Young Materials Scientist","WMRIF - 3rd International Workshop for Young Materials Scientist","2012-08-28","","jpn","oral_presentation","","","","","","","Magnesium alloys have been investigated as a potential bioabsorbable orthopedic metallic material owing to their biodegradability and mechanical properties similar to bone. However, poor corrosion resistance of the alloys results in a too rapid degradation of mechanical integrity in vivo. We developed a single-step solution treatment for hydroxyapatite (HAp) and octacalcium phosphate coatings for pure magnesium and AZ series magnesium alloys to control the corrosion (degradation) rate and to improve their biocompatibility. In this study, microstructure of the HAp coating was analyzed and the corrosion behavior, cytocompatibility and fatigue behavior of HAp-coated AZ31 alloy were examined. " "Hydroxyapatite Coating by Chemical Deposition for a Bioabsorbable Magnesium Alloy","Hydroxyapatite Coating by Chemical Deposition for a Bioabsorbable Magnesium Alloy","廣本 祥子, 友澤 方成, 竹村 太郎, 花方 信孝, 丸山 典夫","廣本 祥子, 友澤 方成, 竹村 太郎, 花方 信孝, 丸山 典夫","NIMS Conference 2012","NIMS Conference 2012","2012-06-04","","jpn","oral_presentation","","","","","","","Magnesium alloys have been investigated as a potential bioabsorbable orthopedic metallic material owing to their degradability in physiological environments and mechanical properties similar to bone. However, very low corrosion resistance of the alloys results in a too rapid degradation of mechanical integrity in vivo. We developed a single-step chemical deposition for hydroxyapatite (HAp) and octacalcium phosphate coatings for pure magnesium and AZ series magnesium alloys to control the corrosion (degradation) rate and to improve their biocompatibility. In this study, microstructure of the HAp coating was analyzed and the corrosion behavior, cytocompatibility and fatigue behavior of HAp-coated AZ31 alloy were examined. " "水酸アパタイト被覆AZ31マグネシウム合金表面でのMG-63細胞の短期培養","水酸アパタイト被覆AZ31マグネシウム合金表面でのMG-63細胞の短期培養","廣本 祥子, 竹村 太郎, 花方 信孝","廣本 祥子, 竹村 太郎, 花方 信孝","日本金属学会2012年春期(第150回)大会","日本金属学会2012年春期(第150回)大会","2012-03-28","","jpn","oral_presentation","","","","","","","マグネシウム合金は生体吸収性金属材料としての応用が期待されているが、生体内での腐食速度が大きすぎることが課題となっている。そこで我々は水熱(化成)処理によるマグネシウムの水酸アパタイト(HAp)被覆法を開発し、HAp被覆純MgおよびAZ31マグネシウム合金が疑似体液中で高い耐食性を示すことを報告した。本研究では、AZ31合金および純MgをHAp被覆し、ヒト骨肉腫由来細胞MG-63を短時間培養して、HAp被覆マグネシウム材の細胞適合性について検討した。AZ31合金および純Mgの両方において、HAp被覆材の方が化学研磨まま材よりも細胞密度が高い傾向がみられた。これより、HAp被覆によりマグネシウム材の細胞適合性が改善することが示唆された。" "Global gene analysis of tisseu resopnse for bioactive/bioinert matrices","皮下に埋入したバイオマテリアル界面に対する生体応答の遺伝子網羅解析","江橋具, 柿木佐知朗, 竹村 太郎, 箕輪 貴司, 花方 信孝, 小林 尚俊, 由井伸彦, 石原一彦, 山岡哲二","江橋具, 柿木佐知朗, 竹村 太郎, 箕輪 貴司, 花方 信孝, 小林 尚俊, 由井伸彦, 石原一彦, 山岡哲二","第32回日本バイオマテリアル学会","第32回日本バイオマテリアル学会","2010-11-26","","jpn","oral_presentation","","","","","","","血管新生は組織再生医療においても重要な要因の一つである。マテリアル移植後の再生に関連する遺伝子を網羅的遺伝子解析の手法を用いて解析し、再生プロセスに関与する重要な遺伝子セットの探求を行っている。今回は、バイオアクティブ材料としてコラーゲン、Bioinert材料としてMPCポリマーを選択肢解析を行った結果を報告する。" "Discovery of Prognostic Factor using Proteomic Analysis with Formalin-Fixed Paraffin-","Discovery of Prognostic Factor using Proteomic Analysis with Formalin-Fixed Paraffin-","高舘達之, 小野川徹, Kiyonaga fujii, 前田晋平, 竹村 太郎, 三上紗弥香, 山田誠子, 福田哲也, 板東泰彦, 碓井史彦, 江川新一, 箕輪 貴司, 花方 信孝, 西村俊秀, 力山敏樹, 片寄 友, 海野倫明","高舘達之, 小野川徹, Kiyonaga fujii, 前田晋平, 竹村 太郎, 三上紗弥香, 山田誠子, 福田哲也, 板東泰彦, 碓井史彦, 江川新一, 箕輪 貴司, 花方 信孝, 西村俊秀, 力山敏樹, 片寄 友, 海野倫明","第14回国際膵臓学会・第41回日本膵臓学会大会","第14回国際膵臓学会・第41回日本膵臓学会大会","2010-07-11","","jpn","oral_presentation","","","","","","","The method for candidate proteomic analysis of FFPE tissue described here offers new opportunities to identify disease-specific biomarkers and therapeutic targets using widely available archival samples with corresponding detailed pathological and clinical records. Furthermore, we aim to identify the effective biomarker using this methodology with blood or biopsy specimen samples in near future." "FFPE組織を用いた網羅的プロテオーム解析による胆道癌新規バイオマーカーの探索","FFPE組織を用いた網羅的プロテオーム解析による胆道癌新規バイオマーカーの探索","前田晋平, 小野川徹, 高舘達之, 森川孝則, 竹村 太郎, 三上紗弥香, 山田誠子, 福田哲也, 板東泰彦, 碓井史彦, 箕輪 貴司, 花方 信孝, 西村俊秀, 力山敏樹, 片寄 友, 江川新一, 海野倫明","前田晋平, 小野川徹, 高舘達之, 森川孝則, 竹村 太郎, 三上紗弥香, 山田誠子, 福田哲也, 板東泰彦, 碓井史彦, 箕輪 貴司, 花方 信孝, 西村俊秀, 力山敏樹, 片寄 友, 江川新一, 海野倫明","第22回日本肝胆膵外科学会・学術集会","第22回日本肝胆膵外科学会・学術集会","2010-05-26","","jpn","oral_presentation","","","","","","","今日の網羅的プロテオーム解析を支える質量分析(マススペクトロメトリー;MS)の技術革新は目覚ましく、微量の試料から高感度で複数のタンパク質を同時定量することが可能となった。ここ数年、臨床医学や薬理学、創薬のための高度医療技術としての応用にも期待され、“臨床プロテオミクス”と呼ばれ注目を集めてきており、なかでも疾患特異的なバイオマーカー探索は、早期診断や治療方針の確立(テーラーメイド医療)、予後や副作用の予測、そして予防医学等、臨床現場への応用が最も期待される分野として競争が激化している。 本研究ではホルマリン固定標本からMSにより胆道癌のマーカーとして利用できる可能性のある数種のタンパク質について紹介する。" "ホルマリン固定パラフィン包埋組織を用いた臨床プロテオミクスによる膵癌新規バイ","ホルマリン固定パラフィン包埋組織を用いた臨床プロテオミクスによる膵癌新規バイ","高舘達之, 小野川徹, 前田晋平, 森川孝則, 竹村 太郎, 元井冬彦, 三上紗弥香, 山田誠子, 福田哲也, 板東泰彦, 碓井史彦, 箕輪 貴司, 花方 信孝, 西村俊秀, 力山敏樹, 片寄 友, 江川新一, 海野倫明","高舘達之, 小野川徹, 前田晋平, 森川孝則, 竹村 太郎, 元井冬彦, 三上紗弥香, 山田誠子, 福田哲也, 板東泰彦, 碓井史彦, 箕輪 貴司, 花方 信孝, 西村俊秀, 力山敏樹, 片寄 友, 江川新一, 海野倫明","第22回日本肝胆膵外科学会・学術集会","第22回日本肝胆膵外科学会・学術集会","2010-05-26","","jpn","oral_presentation","","","","","","","998?2007年までに膵切除が行われ、組織学的に浸潤性膵管癌と確定診断されたFFPE組織156例を対象としてプロテオーム解析を行い、予後不良群:1047種類、予後良好群:1037種類、正常膵管:979種のタンパク質を同定した。胆道癌と同様の手法を用いて群間で発現に有意差のあるタンパク質180種類を選定した。さらにそれぞれのタンパク質についてGene Ontology解析及び文献検索を行い、予後不良群5例と予後良好群5例との比較で8種類、正常膵管5例と膵癌10例の比較で23種類を候補として選定した。 " "病院保管のホルマリン固定化永久組織からプロテオーム解析はどこまでできるか?","病院保管のホルマリン固定化永久組織からプロテオーム解析はどこまでできるか?","小野川徹, 前田晋平, 高舘達之, 竹村 太郎, 三上紗弥香, 福田哲也, 山田誠子, 板東泰彦, 碓井史彦, 箕輪 貴司, 花方 信孝, 西村俊秀, 森川孝則, 元井冬彦, 力山敏樹, 片寄 友, 江川新一, 海野倫明","小野川徹, 前田晋平, 高舘達之, 竹村 太郎, 三上紗弥香, 福田哲也, 山田誠子, 板東泰彦, 碓井史彦, 箕輪 貴司, 花方 信孝, 西村俊秀, 森川孝則, 元井冬彦, 力山敏樹, 片寄 友, 江川新一, 海野倫明","第22回日本肝胆膵外科学会・学術集会","第22回日本肝胆膵外科学会・学術集会","2010-05-26","","jpn","oral_presentation","","","","","","","これまで手術で切除した組織のホルマリン固定組織からプロテオーム研究を行うことは困難であった。ごく最近になり、FFPE組織からのタンパク抽出法が模索・開発され、ホルマリンにより化学的に架橋され脆弱と思われていたタンパク質の保存状態は想像以上に良いことから、従来のプロテオミクスの意表を突く展開が見えつつある。また、質量分析装置の技術革新も目覚ましく、高感度かつ高再現性プロテオーム解析が可能となり、三連四重極型質量分析計によるMRM定量アッセイ(Multiple Reaction Monitoring MS-based assay)は、数十amolに迫る低濃度での高感度検出を可能とし、多数のターゲットを同時に定量解析することが出来る極めてスループットの高い測定方法として、プロテオミクスにおける“Proof-of-Concept”の新手法として注目されている。 本発表ではホルマリン固定切片のプロテオーム技術について紹介する。" "Gene expression analysis of early host response against scaffold ","スキャフォールド材料に対する生体反応の遺伝子網羅的解析","江橋 具, 竹村 太郎, 箕輪 貴司, 花方 信孝, 小林 尚俊, 山岡 哲二","江橋 具, 竹村 太郎, 箕輪 貴司, 花方 信孝, 小林 尚俊, 山岡 哲二","第31回日本バイオマテリアル学会大会","第31回日本バイオマテリアル学会大会","2009-11-16","","jpn","oral_presentation","","","","","","","各種医療材料に対する生体反応を検索するために、遺伝子の網羅的解析を行った結果を発表する。" "Initial adhesion behavior of fibroblasts onto hydroxyapatite nano-crystals","繊維芽細胞の水酸アパタイトナノ結晶への初期接着挙動","多賀谷 基博, 生駒 俊之, 竹村 太郎, 奥田 充宏, 吉岡朋彦, 花方 信孝, 田中順三","多賀谷 基博, 生駒 俊之, 竹村 太郎, 奥田 充宏, 吉岡朋彦, 花方 信孝, 田中順三","22nd International Symposium on Ceramics in Medicine","22nd International Symposium on Ceramics in Medicine","2009-10-26","","jpn","oral_presentation","","","","","","","Hydroxyapatite (HAp) shows a good biocompatibility for fibroblasts; down growth of percutaneous device composed of HAp sintered body [1] and catheter of HAp coating [2] can be improved. The characteristic features are attributed to the surface property of HAp. Understanding protein adsorption and cell adhesion onto HAp surface is of importance for controlling cell functions. To detect the initial behavior at meso-region, quartz crystal microbalance with dissipation (QCM-D) technique is one of excellent in situ analytical methods. In this study, the QCM-D technique was employed for the initial adhesion and spreading of fibroblast NIH3T3 cells onto HAp nanosensor." "Hepatocyte adhesion behavior on modified hydroxyapatite nanocrystals with quartz crystal microbalance","QCM-Dによる水酸アパタイトナノ結晶への肝細胞の接着挙動","生駒 俊之, 多賀谷 基博, 山崎 智彦, 竹村 太郎, 花方 信孝","生駒 俊之, 多賀谷 基博, 山崎 智彦, 竹村 太郎, 花方 信孝","22nd International Symposium on Ceramics in Medicine","22nd International Symposium on Ceramics in Medicine","2009-10-26","","jpn","oral_presentation","","","","","","","Hydroxyapatite shows the mild protein adsorption [1] and the unique cell adhesion behavior. The main applications of hydroxyapatite are focused on the bone filling materials and scaffold for bone tissue engineering. In the present study, we expand the hydroxyapatite applications to the cell culture substrate for the hepatocyte-like cells. The adsorption behavior of collagen (Col) and fibronectin (FN) and subsequent adsorption of fetal bovine serum (FBS) and hepatocyte adhesion on the modified hydroxyapatite surfaces were investigated with quartz crystal microbalance with dissipation technique (QCM-D)." "チタン、ジルコニウム、金における骨芽細胞様細胞骨分化特性の網羅的遺伝子発現解析","チタン、ジルコニウム、金における骨芽細胞様細胞骨分化特性の網羅的遺伝子発現解析","大家渓, 竹村 太郎, 花方 信孝, 森山義之, 野田和彦, 堤祐介, 土居壽, 野村直之, 塙隆夫","大家渓, 竹村 太郎, 花方 信孝, 森山義之, 野田和彦, 堤祐介, 土居壽, 野村直之, 塙隆夫","日本金属学会2009年秋期講演大会","日本金属学会2009年秋期講演大会","2009-09-15","","jpn","oral_presentation","","","","","","","DNAマイクロアレイによる測定から骨芽細胞の石灰化に関与している遺伝子の発現はZrやAu上で骨分化を誘導した細胞と比較してTiの方が高かった。これらの結果から遺伝子レベルではTiはZrやAuに比較して硬組織適合性が優れているとこが判明した。" "Discovery proteomics using formain-fixed paraffin-embedded tissues of cholangiocarcinoma","胆道癌ホルマリン固定パラフィン包埋組織切片からのディスカバリープロテオミクス","小野川徹, 前田晋平, 岡上能斗竜, 高舘達之, 石田和之, 前田 めぐみ, 竹村 太郎, 三上紗弥香, 遠藤洋子, 山田誠子, 西山隆太郎, 箕輪 貴司, 花方 信孝, 板東泰彦, 碓井史彦, 西村俊秀, 力山敏樹, 片寄友, 江川新一, 海野倫明","小野川徹, 前田晋平, 岡上能斗竜, 高舘達之, 石田和之, 前田 めぐみ, 竹村 太郎, 三上紗弥香, 遠藤洋子, 山田誠子, 西山隆太郎, 箕輪 貴司, 花方 信孝, 板東泰彦, 碓井史彦, 西村俊秀, 力山敏樹, 片寄友, 江川新一, 海野倫明","日本ヒトプロテオーム機構(JHUPO)第7回大会","日本ヒトプロテオーム機構(JHUPO)第7回大会","2009-07-27","","jpn","oral_presentation","","","","","","","胆道癌組織に特異的に発現する分泌タンパク質をレーザーマイクロダイセクッションおよび質量分析により探索し、その候補タンパク質を抽出した。" "Monitoring of Adsorption of Multiple Proteins onto Hydroxyapatite Nanosensor by Quartz Crystal Microbalance with Dissipation Technique","複数タンパク質の水酸アパタイトナノセンサーへの吸着挙動のQCM-Dによる計測","多賀谷基博, 奥田充宏, 生駒俊之, 竹村太郎, 吉岡朋彦, 花方信孝, 田中順三","多賀谷基博, 奥田充宏, 生駒俊之, 竹村太郎, 吉岡朋彦, 花方信孝, 田中順三","The Third International Conference on the Science and Technology","The Third International Conference on the Science and Technology","2009-06-16","","eng","oral_presentation","","","","","","The interfacial protein layers between biomaterials and biological tissues govern the biocompatibility [1]. Therefore, understanding of the protein-surface interaction is important for designing superior biocompatible materials. In this study, multiple protein adsorption of fetal bovine serum (FBS) onto a hydroxyapatite (HAp) nanosensor was in situ analyzed in different solvents by a quartz crystal microbalance with dissipation (QCM-D) technique.","" "Discovery proteomics using formain-fixed paraffin-embedded tissues of cholangiocarcinoma","胆道癌ホルマリン固定パラフィン包埋(FFPE)組織からのディスカバリープロテオミクス","小野川徹, 前田晋平, 岡上能斗竜, 森川孝則, 高舘達之, 石田和之, 前田 めぐみ, 竹村 太郎, 三上紗弥香, 遠藤洋子, 山田誠子, 西山隆太郎, 箕輪 貴司, 花方 信孝, 板東泰彦, 碓井史彦, 西村俊秀, 力山敏樹, 片寄友, 江川新一, 海野倫明","小野川徹, 前田晋平, 岡上能斗竜, 森川孝則, 高舘達之, 石田和之, 前田 めぐみ, 竹村 太郎, 三上紗弥香, 遠藤洋子, 山田誠子, 西山隆太郎, 箕輪 貴司, 花方 信孝, 板東泰彦, 碓井史彦, 西村俊秀, 力山敏樹, 片寄友, 江川新一, 海野倫明","第5回 日本臨床プロテオーム研究会2009","第5回 日本臨床プロテオーム研究会2009","2009-05-09","","jpn","oral_presentation","","","","","","","胆道癌組織からレーザーマイクロダイセクションに切り出した癌細胞のタンパク質発現を質量分析により同定し、正常組織と比較することによって癌マーカーの探索を行い、候補タンパク質を抽出した。" "A novel osteoblast-specific gene, Ifitm5","骨芽細胞特異的な新奇遺伝子Ifitm5","森田 浩美, 竹村 太郎, リ コウラン, 箕輪 貴司, 花方 信孝","森田 浩美, 竹村 太郎, リ コウラン, 箕輪 貴司, 花方 信孝","第31回日本分子生物学会年会","第31回日本分子生物学会年会","2008-12-09","","jpn","oral_presentation","","","","","","","この遺伝子の発現はMC3T3-E1細胞の培養において石灰化期に誘導され、BMP-2によって発現が促進された。一方、破骨細胞分化促進因子であるIL-1αはこの遺伝子の発現を阻害した。マウス胚を用いたin situハイブリダイゼーション実験から、Ifitm5は骨組織のみにE14.5から発現が認められ、軟骨性骨化および膜性骨化の両方に関与することが明らかとなった。また、軟骨細胞での発現は観察されなかった。これらの結果から、Ifitm5は骨芽細胞特異的で骨芽細胞の分化あるいは石灰化に関与していることが示唆された。そこで、骨芽細胞の分化に重要な転写因子であるCbfa1/Runx2およびOsterixによるIfitm5の発現制御について検討を行ったが、これら2つの転写因子の関与は認められなかった。" "エンテロウイルス71の感染性決定分子の同定","エンテロウイルス71の感染性決定分子の同定","山吉誠也, 山下康子, 花方 信孝, 箕輪 貴司, 竹村 太郎, 清水博之, 小池智","山吉誠也, 山下康子, 花方 信孝, 箕輪 貴司, 竹村 太郎, 清水博之, 小池智","第56回 日本ウイルス学会学術集会","第56回 日本ウイルス学会学術集会","2008-10-26","","jpn","oral_presentation","","","","","","","エンテロウイルス71(EV71)は手足口病の原因ウイルスであり、稀に重篤な無菌性髄膜炎および脳炎を引き起こすこともある。我々はEV71の神経病原性発現機構を解明することを目的として、まずEV71の感染受容体の同定を試みている。本研究によりL051細胞にEV71感染性をもたらしているRD-A細胞由来の分子をDNAマイクロアレイによって明らかにした。感染成立にはこの一つの分子をL929細胞に導入するだけで十分であり、用いた4株の感染が成立し得たことからEV71すべての株に共通の受容体であると考えられた。" "Adsorption of Proteins Derived from Fetal Bovine Serum onto Hydroxyapatite Nanocrystals with Quartz Crystal Microbalance Techniq","QCM-Dを用いたウシ胎児血清からの水酸アパタイトへのタンパク質の吸着","多賀谷 基博, 生駒 俊之, 竹村 太郎, 奥田 充宏, 花方 信孝","多賀谷 基博, 生駒 俊之, 竹村 太郎, 奥田 充宏, 花方 信孝","21st International Symposium of Ceramics in Medicine","21st International Symposium of Ceramics in Medicine","2008-10-21","","jpn","oral_presentation","","","","","","","The adsorption of multiple proteins derived from fetal bovine serum (FBS) in phosphate buffer saline (PBS) and alpha minimum essential (alpha MEM) was in situ analyzed with a quartz crystal microbalance with dissipation technique on gold, titanium and HAp sensors. The D/f value of the HAp sensor were clearly different in PBS and alpha MEM, and others were not changed. The D-f plots incidated that the proteins adsorbed on HAp in PBS would lead to competitive adsorption and conformational change and those in alpha MEM could form a monolayer. The adsorption behavior on the HAp in carbonate buffer saline was found to be similar to that in alpha MEM. These differential adsorption behaviors on the HAp surface were attributed to the pre-adsorptive ion, such PO43- or CO32- in the solvent." "Evaluation of Mutagenicity of Static Magnetic Fields up to 13 T using E-Coli deficient in ROS removal function","活性酸素除去機能を欠損する大腸菌による強磁場13 Tの変異原性評価","吉江幸子, 池畑政輝, 早川敏雄, 廣田 憲之, 竹村 太郎, 箕輪 貴司, 花方 信孝","吉江幸子, 池畑政輝, 早川敏雄, 廣田 憲之, 竹村 太郎, 箕輪 貴司, 花方 信孝","日本磁気科学会第3回年次大会","日本磁気科学会第3回年次大会","2008-10-01","","jpn","oral_presentation","","","","","","","最大13Tの磁場を用いて、活性酸素除去機能を欠損する大腸菌の変異原性を評価した結果について報告する" "Analysis of Removal of Protein Adsorbed on Hydroxyapatite Nano-crystals by Quartz Crystal Microbalance","QCM-Dを用いた水酸アパタイトナノ結晶表面へ吸着したタンパク質の除去の解析","多賀谷基博, 生駒俊之, 竹村太郎, 奥田充宏, 花方信孝","多賀谷基博, 生駒俊之, 竹村太郎, 奥田充宏, 花方信孝","第21回セラミックス協会秋季シンポジウム","第21回セラミックス協会秋季シンポジウム","2008-09-17","","jpn","oral_presentation","","","","","","","我々は、生体組織が接する材料表面での界面親和性の解明を目的とし、水酸アパタイト (HAp) 表面へのタンパク質の吸着をQuartz Crystal Microbalance with Dissipation (QCM-D) により検討している。本研究では、HApセンサー表面へ吸着したタンパク質の過酸化水素水とアンモニアとの混合液 (APM) や低圧水銀ランプ由来の紫外線 (UV) による除去について検討した。原子間力顕微鏡から、センサー表面の粗さや形態は洗浄によって変化しないことを確認した。QCM-Dから、APMではタンパク質の除去が不完全で、UVを組み合わせることで除去できることがわかった。" "Evaluation of mutagenicity of static magnetic field up to 13T using E. coli deficient in ROS removal function","活性酸素除去機能を欠損する大腸菌による強磁場13Tの変異原性評価","吉江幸子, 池畑政輝, 早川敏雄, 廣田 憲之, 竹村 太郎, 箕輪 貴司, 花方 信孝","吉江幸子, 池畑政輝, 早川敏雄, 廣田 憲之, 竹村 太郎, 箕輪 貴司, 花方 信孝","第32回日本磁気学会学術講演会","第32回日本磁気学会学術講演会","2008-09-12","","jpn","oral_presentation","","","","","","","近年の定常磁場の生物影響に関する研究において,酸化ストレスの付与や活性酸素種の寿命への効果を示唆する報告等がなされている。これらの報告のうち,Zhangらは,Superoxide dismutase (SOD)遺伝子を欠損する大腸菌において,磁場強度と相関して遺伝子変異頻度が顕著に上昇することを報告している。我々は,これまでに,同じ大腸菌株を5Tの定常磁場に曝露し,Zhangらとは異なる指標を用いて遺伝子変異頻度を検討したところ,磁場の変異原性は観察されないという結果を得ている。本研究では,同株の遺伝子変異頻度に関して13Tまでの定常強磁場への曝露による影響を評価した。" "Stimulation of Osteoblast Maturation by Acid Treated Hydroxyapatite Surface","Stimulation of Osteoblast Maturation by Acid Treated Hydroxyapatite Surface","竹村 太郎, 生駒 俊之, 花方 信孝","竹村 太郎, 生駒 俊之, 花方 信孝","1st Asian Biomaterials Congress","1st Asian Biomaterials Congress","2007-12-06","","jpn","oral_presentation","","","","","","","Not only hydrochloric acid treatment but also nitric acid treatment improved the performance of HAp to mature osteoblast. To high performance, it may be important that the surface of HAp has the specific shape, which is extremely rough and has many small single HAp crystals." "Stem Cell Gene Expression on Cord Blood Cells with the Lapse of Time after Harvest","Stem Cell Gene Expression on Cord Blood Cells with the Lapse of Time after Harvest","高森靖, 鈴木恵子, 浅井芳明, 野垣兼朗, 竹村 太郎, 花方 信孝","高森靖, 鈴木恵子, 浅井芳明, 野垣兼朗, 竹村 太郎, 花方 信孝","第37回日本免疫学会総会","第37回日本免疫学会総会","2007-11-20","","jpn","oral_presentation","","","","","","","We investigated the quality of CBMCs through the examine on the stem cells gene expression. The stem cell marker genes (Oct4, Nanog, Sox-2) were expressed likewise, but the pattern was different from the cases of Bmi-1 and ZFX. As CBMCs (within 48h after harvest) express stem cell genes and self-renewal associated genes, CBMCs are considered functional and useful for regenenerative medicine and cell therapy as well as conventional hematopoietic stem cell transplantation." "Effects of Static Magnetic Fields on Escherichia coli deficient in superoxide dismutase","Effects of Static Magnetic Fields on Escherichia coli deficient in superoxide dismutase","吉江幸子, 池畑政輝, 廣田 憲之, 竹村 太郎, 箕輪 貴司, 花方 信孝, 早川敏雄","吉江幸子, 池畑政輝, 廣田 憲之, 竹村 太郎, 箕輪 貴司, 花方 信孝, 早川敏雄","The International Conference on Magneto-Science ICMS2007","The International Conference on Magneto-Science ICMS2007","2007-11-11","","jpn","oral_presentation","","","","","","","Superoxide dismutase遺伝子を欠損する大腸菌を用いて、最大13 Tの磁場の下、静磁場の変異原性を評価した結果について報告する。" "Effect of Acid Treated Hydroxyapatite on Osteoblast Maturation","Effect of Acid Treated Hydroxyapatite on Osteoblast Maturation","竹村太郎","竹村太郎","BIOCERAMICS 20","BIOCERAMICS 20","2007-10-24","","eng","oral_presentation","","","","","","Surfaces of sintered hydroxyapatite (HAp) were treated with four kinds of acids, hydrochloric acid (HCl), nitric acid (HNO3), sulfuric acid (H2SO4) and hydrogen peroxide (H2O2). On these acid-treated HAp surfaces, osteoblast-like MC3T3-E1 cells were cultured for 20 days. The matrix mineralization was observed in HCl-treated HAp and HNO3-treated HAp, but not in non-treated HAp, H2SO4-treated HAp and H2O2-treated HAp. Gene expressions of Bglap2 encoding osteocalcin and Akp2 encoding alkaline phosphatase were higher in HCl-treated HAp and HNO3-treated HAp than those in the others. These results indicate that not only hydrochloric acid but also nitric acid improves performance of HAp to mature osteoblast.","" "Cell adhesion and structural analysis of polymer-protein composite nanofiber","高分子-タンパク混紡型ナノファイバーの細胞接着性と構造解析","横山 敬郎, 竹村 太郎, 花方 信孝, 小林 尚俊","横山 敬郎, 竹村 太郎, 花方 信孝, 小林 尚俊","第56回 高分子学会年次大会","第56回 高分子学会年次大会","2007-05-29","","jpn","oral_presentation","","","","","","","我々はナノファイバーの特性を考慮し、新規再生医工学足場材料の研究に取り組んでいる。細胞-材料複合体を生体内に埋入して早期生着、長期機能維持をさせるには血管網の構築が必須であり血管誘導能を有する足場材料が求められている。我々は、生体適合性、機械的強度に優れ臨床現場で使用実績のあるポリグリコール酸(PGA)の血管誘導材料としての機能化に取り組み、エレクトロスピニング法によって作製されたPGA-コラーゲン混紡型ナノファイバーは、良好な細胞接着性を有することを示してきた。本研究では、同ファイバーの構造解析及び物性評価を行い、細胞接着性との相関を検討した。" "Enhanced expression of the osteoblastic phenotype by hydrochloric acid treated hydroxyapatite","Enhanced expression of the osteoblastic phenotype by hydrochloric acid treated hydroxyapatite","竹村太郎","竹村太郎","Asian BioCeramic Symposium 2006","Asian BioCeramic Symposium 2006","2006-11-07","","eng","oral_presentation","","","","","","We examined the effect of HCl treatment of sintered hydroxyapatite (HAp) surface on osteoblast maturation. Sintered HAp with polished mirror surface was treated with 0.1M HCl for 5 min. Mouse osteoblast-like MC3T3-E1 cells were cultured on non-treated and HCl-treated HAp surfaces for 20 days. The matrix mineralization was observed in HCl-treated HAp surface, but not in non-treated HAp surface. The expression levels of specific marker genes, Akp2 encoding alkaline phosphatase and Bglap2 encoding osteocalcin, were 1.4 and 2.7 times higher in HCl-treated HAp surface than in non-treated HAp surface. These results indicate that HCl treatment of HAp surface promotes osteoblast maturation. Although the HCl treatment increased surface roughness of HAp, laser microscopic observation suggests that c","" "Gene expression profile of osteoblast-like cells on calcium phosphate biomaterials","Gene expression profile of ostbleoast-like cells on calcium phosphate biomaterials","竹村太郎","竹村太郎","BIOCERAMICS 19","BIOCERAMICS 19","2006-10-10","","eng","oral_presentation","","","","","","Gene expression profile of osteoblast-like cells cultured on dense disk materials and porous materials of calcium phosphate ceramics was constructed from DNA microarray analyses. The profile revealed that gene expression patterns of porous materials were significantly different from those of dense disk materials. The porous materials had a capacity to induce expressions of genes involved in osteoblast differentiation, while dense disk materials regulated gene expressions related to induction of osteoclastogenesis.",""